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ncing reaction was performed with ABI 3730 DNA Sequencer and BigDye Terminator v3.1 Cycle Sequencing Kit as previously described. Resulting chromatograms were base-called with in-house software RECall and aligned to a modified HXB2 V3-loop reference. All Sanger sequences were deposited into GenBank. For ��deep��sequencing, samples were put through triplicate reverse transcription reactions and ��n